5'-tailed sequencing primers improve sequencing quality of PCR products.
نویسندگان
چکیده
منابع مشابه
The use of tailed octamer primers for cycle sequencing.
Studies have been carried out on the use of octamer oligonucleotides tailed with different base analogues as primers in cycle sequencing reactions. 5-Nitroindole tails improved the performance as primers of a number of octamers. A tail length of three or four 5-nitroindole residues significantly increased the sequencing signal intensity for almost all primers. The use of incomplete libraries of...
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ورودعنوان ژورنال:
- BioTechniques
دوره 42 2 شماره
صفحات -
تاریخ انتشار 2007